rat hsp 90 elisa kit  (Novus Biologicals)

Journal: PLoS ONE

Article Title: Biological Responses of Three-Dimensional Cultured Fibroblasts by Sustained Compressive Loading Include Apoptosis and Survival Activity

doi: 10.1371/journal.pone.0104676

Figure Lengend Snippet: Fibroblasts were seeded to collagen sponge and incubated for 24(□) or 200 mmHg (▪) compression for 6 h. Total mRNA was extracted after WST-1 assay, and mRNA expression was assessed using real-time RT-PCR. The expression of the target genes in the 6 h–200 mmHg group relative to the value in the 6 h–0 mmHg group was calculated by the comparative Ct method using the 18S ribosomal RNA gene as an internal control. The results are represented as the mean ± SEM (error bars) of five experiments. Statistical analysis was performed using the Student’s t test between the 0 mmHg group and the 200 mmHg group, and statistical significance was taken as p <0.05. A value of p was expressed as: *; p <0.05, **; p <0.01, and ***; p <0.001. A: The transcription factors of various Hsps . B: various Hsps C: Bcl2 is an antiapoptotic gene, and Bax is a proapoptotic gene. D and E: Immunostaining for HSP90α. Representative sections of (D) the 6 h–0 mmHg group and (E) the 6 h–200 mmHg group. Higher expression and nucleus translocation of HSP90α was observed in the 200 mmHg group (E) when compared with the 0 mmHg group (D). Scale bars = 20 µm for all images.

Article Snippet: HSP90α concentration was measured using the Rat Heat Shock Protein 90α ELISA kit (CUSABIO BIOTECH, Wuhan, China).

Techniques: Incubation, WST-1 Assay, Expressing, Quantitative RT-PCR, Control, Immunostaining, Translocation Assay

Journal: PLoS ONE

Article Title: Biological Responses of Three-Dimensional Cultured Fibroblasts by Sustained Compressive Loading Include Apoptosis and Survival Activity

doi: 10.1371/journal.pone.0104676

Figure Lengend Snippet: Fibroblasts were seeded to collagen sponge and incubated for 24(A: HSP90α, B: HA, C: PGE 2 ) was measured by ELISA. A value of concentration was normalized by WST-1 value. The results are represented as the mean ± SEM (error bars) of five experiments. Statistical analysis was performed using the Dunnett’s multiple test between non-loaded group and each of loaded group, and statistical significance was taken as p <0.05. A value of p was expressed as: *; p <0.05, **; p <0.01, and ***; p <0.001.

Article Snippet: HSP90α concentration was measured using the Rat Heat Shock Protein 90α ELISA kit (CUSABIO BIOTECH, Wuhan, China).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Concentration Assay